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alp  (R&D Systems)


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    Structured Review

    R&D Systems alp
    Alp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alp+antibody/Human+Alkaline+Phosphatase%2FALPL+Antibody/pm41961374-109-25-26
    Average 94 stars, based on 9 article reviews
    alp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Lymphotoxin-β promotes breast cancer bone metastasis colonization and osteolytic outgrowth.
    Article Snippet: Bone metastasis is a lethal consequence of breast cancer.. Here we used single-cell transcriptomics to investigate the molecular mechanisms underlying bone metastasis colonization—the rate-limiting step in the metastatic cascade.. We identified that lymphotoxin-β (LTβ) is highly expressed in tumour cells within the bone microenvironment and this expression is associated with poor bone metastasis-free survival.

    Article Title: Ex vivo 3D osteocyte network construction with primary murine bone cells
    Article Snippet: .. After blocking cells were then incubated overnight at 4 °C with a goat anti-mouse sclerostin antibody (affinity purified polyclonal antibody, RnD systems, MN, USA) or ALP antibody (RnD systems) followed by secondary staining with a fluorescence-conjugated antibody (rhodamine AffiniPure rabbit anti-goat IgG, Jackson ImmunoResearch Laboratories, PA, USA). .. ALP expression was also tested by TRACP&ALP double-stain Kit (TaKaRa, Shiga, Japan).

    Article Title: Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity
    Article Snippet: .. The samples were stained with hematoxylin–eosin or ALP antibody (R&D System) overnight. ..

    Blocking Assay:

    Article Title: Ex vivo 3D osteocyte network construction with primary murine bone cells
    Article Snippet: .. After blocking cells were then incubated overnight at 4 °C with a goat anti-mouse sclerostin antibody (affinity purified polyclonal antibody, RnD systems, MN, USA) or ALP antibody (RnD systems) followed by secondary staining with a fluorescence-conjugated antibody (rhodamine AffiniPure rabbit anti-goat IgG, Jackson ImmunoResearch Laboratories, PA, USA). .. ALP expression was also tested by TRACP&ALP double-stain Kit (TaKaRa, Shiga, Japan).

    Incubation:

    Article Title: Ex vivo 3D osteocyte network construction with primary murine bone cells
    Article Snippet: .. After blocking cells were then incubated overnight at 4 °C with a goat anti-mouse sclerostin antibody (affinity purified polyclonal antibody, RnD systems, MN, USA) or ALP antibody (RnD systems) followed by secondary staining with a fluorescence-conjugated antibody (rhodamine AffiniPure rabbit anti-goat IgG, Jackson ImmunoResearch Laboratories, PA, USA). .. ALP expression was also tested by TRACP&ALP double-stain Kit (TaKaRa, Shiga, Japan).

    Affinity Purification:

    Article Title: Ex vivo 3D osteocyte network construction with primary murine bone cells
    Article Snippet: .. After blocking cells were then incubated overnight at 4 °C with a goat anti-mouse sclerostin antibody (affinity purified polyclonal antibody, RnD systems, MN, USA) or ALP antibody (RnD systems) followed by secondary staining with a fluorescence-conjugated antibody (rhodamine AffiniPure rabbit anti-goat IgG, Jackson ImmunoResearch Laboratories, PA, USA). .. ALP expression was also tested by TRACP&ALP double-stain Kit (TaKaRa, Shiga, Japan).

    Fluorescence:

    Article Title: Ex vivo 3D osteocyte network construction with primary murine bone cells
    Article Snippet: .. After blocking cells were then incubated overnight at 4 °C with a goat anti-mouse sclerostin antibody (affinity purified polyclonal antibody, RnD systems, MN, USA) or ALP antibody (RnD systems) followed by secondary staining with a fluorescence-conjugated antibody (rhodamine AffiniPure rabbit anti-goat IgG, Jackson ImmunoResearch Laboratories, PA, USA). .. ALP expression was also tested by TRACP&ALP double-stain Kit (TaKaRa, Shiga, Japan).

    Recombinant:

    Article Title: Germinated soy germ with increased soyasaponin Ab improves BMP-2-induced bone formation and protects against in vivo bone loss in osteoporosis
    Article Snippet: .. Recombinant human bone morphogenetic protein-2 (rhBMP-2) and ALP antibody were purchased from R&D Systems (Minneapolis, MN). .. Penicillin, streptomycin, cell culture medium, and fetal bovine serum (FBS) were purchased from Invitrogen Life Technologies (Carlsbad, CA).

    Control:

    Article Title: Down-expression of miR-494-3p in senescent osteocyte-derived exosomes inhibits osteogenesis and accelerates age-related bone loss via PTEN/PI3K/AKT pathway
    Article Snippet: Next, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology) for 1.5 hours at room temperature. .. The primary antibodies used were P16 (1:1000, ab51243; Abcam, USA), Runt-related transcription factor 2 (Runx2, 1:1,000, 12,556 S; Cell Signaling Technology), collagen type I α1 (Col-I, 1:1,000, 72,026 T; Cell Signaling Technology), ALP antibody (1:1,000, AF2910; R&D Systems, USA), AKT (1:1,000, 60203-2-Ig; Proteintech, USA), phosphorylated (p)-AKT (1:1,000, 66444-1-Ig; Proteintech), PI3K (1:1,000, 20584-1-AP; Proteintech), PTEN (1:1,000, 22034-1- AP; Proteintech), CD9 (1:1,000, 60232-1-Ig; Proteintech), and CD63 (1:1,000, 67605-1-Ig; Proteintech), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:5,000, 60004-1-Ig; Proteintech) was used as a loading control. .. The signals were visualized with an enhanced chemiluminescence (ECL) detection reagent (P0018FM; Beyotime) and semi-quantified with ImageJ software (version 1.53 n).

    Article Title: Down-expression of miR-494-3p in senescent osteocyte-derived exosomes inhibits osteogenesis and accelerates age-related bone loss via PTEN/PI3K/AKT pathway
    Article Snippet: Next, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology) for 1.5 hours at room temperature. .. The primary antibodies used were P16 (1:1000, ab51243; Abcam, USA), Runt-related transcription factor 2 (Runx2, 1:1,000, 12,556 S; Cell Signaling Technology), collagen type I α1 (Col-I, 1:1,000, 72,026 T; Cell Signaling Technology), ALP antibody (1:1,000, AF2910; R&D Systems, USA), AKT (1:1,000, 60203-2-Ig; Proteintech, USA), phosphorylated (p)-AKT (1:1,000, 66444-1-Ig; Proteintech), PI3K (1:1,000, 20584-1-AP; Proteintech), PTEN (1:1,000, 22034-1-AP; Proteintech), CD9 (1:1,000, 60232-1-Ig; Proteintech), and CD63 (1:1,000, 67605-1-Ig; Proteintech), while glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:5,000, 60004-1-Ig; Proteintech) was used as a loading control. .. The signals were visualized with an enhanced chemiluminescence (ECL) detection reagent (P0018FM; Beyotime) and semi-quantified with ImageJ software (version 1.53 n).



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